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Cell culture and cytotoxicity determination HK-2 cells purchased from the Wuhan Procell Life Technology Co., LTD were cultured in Dulbeccos modified eagle medium (DMEM) with glucose at a concentration of 4.5 g/L (Gibco, USA), supplemented with 10% fetal bovine serum (Sijiqing, China) and 100 U/mL penicillin-streptomycin (Gibco, USA) according to the handling procedure (Zhou, 2020)

[DOI] [PMC free article] [PubMed] [Google Scholar] 50.Zhao H., Fu W., Yang X., Zhang W., Wu S., Ma J., Zhang T., Yao H., Zhang Z

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By inhibiting the enzyme, the skin no longer produces pigmentation

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doi: 10.1186/s12967-025-06315-7