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Description
The molecular mechanism of autophagy

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Immunoblot assays Tissue samples were crushed on dry ice to obtain homogenous aliquots and then lysed in RIPA buffer (Thermo Scientific #89900) containing Halt protease & phosphatase inhibitor (Thermo Scientific #1861280)
105 (suppl 2): 417436

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Firstly, due to its revolutionary mechanism of action, in which the suppression of the biological effect is obtained through the degradation of the therapeutic target and protein aggregates, using the cells own machinery 20