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Insert the needle into the rubber stopper and push in the air into the vial, this creates a vacuum-like seal

We are aware that some of the subjects may have had genetically low concentrations of transcobalamin I (TCI), which is now known to be a benign condition that most probably does not need medical treatment [17]

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The use of blood concentrations of vitamins and their respective functional indicators to define folate and vitamin B12 status