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Cells were lysed with RIPA buffer, and the lysates were subjected to SDS-PAGE and visualized by Western blotting using antibodies against PGRMC1, EGFR, phospho-Y1068 EGFR (Cell signaling: #2234S), AKT (Cell signaling: #9272S), phospho-S473AKT (Cell signaling: #4060S), ERK (Cell signaling: #4695S) and phospho-T185 Y187 ERK (Invitrogen: 44680 G)

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Furthermore, these chosen strains exhibited notable vitality throughout fermentation and storage of fermented milk at 4 C

Dilution Ratio: A common starting point is 5 drops of tea tree oil per teaspoon of carrier oil, but this can be adjusted based on individual sensitivity