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Interestingly, UPR is also activated in neurodegenerative tauopathies such as AD

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Once internal standard concentrations were obtained, the peak areas of the unlabelled metabolites in the samples of culture medium were compared with the peak area of the quantified internal standard to determine the metabolite concentration in the sample

[DOI] [PubMed] [Google Scholar] Porath J., Carlsson J.A.N., Olsson I., Belfrage G

Cell Death Dis 4:e470
8 h post-transfection, medium was changed into 2 ml of DMEM (Gibco, 41966-052) supplemented with 10% (v/v) FCS