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They're process gaps

Thioredoxin-interacting protein (TXNIP), a suppressor of glucose uptake known to be active in low-glutamine conditions as a glycolytic sensor, was found to be strongly expressed in the glutamine-deprived setting in activated CD8+ T cells [151]

We then converted the tryptoline acrylamide-N112C-CCNE1 interaction into a NanoBRET-ABPP assay capable of identifying compounds that reversibly inhibit both N112C- and WT-CCNE1:CDK2 complexes

Commonly used reducing agents include TCEP, DTT, and bME

What a high B12 means depends on the rest of the picture: the timing of the last oral or injected dose, the patient's symptoms, the examination, and the other laboratory results