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Description
Pyruvate kinase deficiency is the most common cause of inherited non-spherocytic hemolytic anemia and the second most common cause of inherited hemolytic anemia behind glucose-6-phosphate dehydrogenase (G6PD) deficiencies

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40, D1178D1186

10.1007/978-1-4684-5931-9_6 Adv

The first-round PCR was performed in five separate 40 l reactions, each containing 2.0 ng of plasmid template DNA, 1 l each of 10 M forward primer KI#403 and 10 M reverse primer KI#404, 0.4 l of Phusion High-Fidelity DNA Polymerase (NEB, no

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