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There is also a growing body of evidence supporting similar membrane domains in prokaryotes, which may depend on sterol-analogous molecules like hopanoids (Saenz et al., 2012

coli mutants also exhibited elevated ROS levels

115118,131 The inward-to-outward open transition itself appears to be driven by the straightening of TM7 (the only helix bridging the N- and C-lobes) coupled to the lowering of the cytoplasmic loop connecting TM6 and TM7