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For genotyping, DNA from toe clipping of littermate animals was extracted using a house-made Lysis buffer (0.1 M Tris-HCl pH 7.5, 0.1 M EDTA, 0.1 M NaCl, 1% SDS), and the PCR was performed using primers described in Supplementary Dataset 5 and the products were analyzed on an agarose gel

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Carey IM, Critchley JA, DeWilde S, Harris T, Hosking FJ, Cook DG

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The Science Behind the Stack The rationale for combining peptides with TRT is rooted in the concept of endocrine synergy different hormonal pathways working together to produce outcomes greater than the sum of their parts