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The next day, the spheroids were washed 3 times with SWB solution for 1 h each at 4 C and incubated with secondary antibodies conjugated with Alexa Fluor 488, TRITC, or Alexa Fluor 647 diluted 1:400 and DAPI (1:10,000) in SWB, overnight at 4 C on an orbital shaker

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it's the molecule your cells rely on to neutralise free radicals, support liver detoxification, and help recycle other antioxidants like vitamin C and vitamin E back into their active forms

writingoriginal draft preparation, T.T., G.B., A.G., Y.S., S.C

Hence, even if bilateral adrenal glands are removed, the remaining amounts secreted prevent death 49,50,51
