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For pathway reconstitution experiments, the codon-optimized BCCT gene (Aci1347) was cloned into plasmid pGEX-6P-1- cntAB using the In-Fusion cloning system (Takara) yielding construct pGEX-6P-1- cntAB-bcct

This leads to a higher concentration of carotenoids in tissues with high oxidative turnover, such as skeletal muscle and liver, with tissue increases of up to 5 times compared to the free form [112,123]

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