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Western blot analysis Rat ONH astrocytes were lysed with lysis buffer (20 mM HEPES pH 7.0, 1% TritonX-100, 10% glycerol, 2 mM EGTA, 1 mM EDTA pH 8.0 (w/NaOH), 20 mM -glycerolphosphate, complete protease inhibitors (Roche Biochemicals, Indianapolis, IN, USA) and phosphatase inhibitors (1 g/ml Aprotinin, 1 g/ml Leupeptin, 1 mM AEBSF, 1 mM Na 3 VO 4 , 5 mM NaF and 2.5 mM Microcystin-LR))