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The results presented are spatial averages over the regions delineated by the dashed boxes in Fig

Beads were washed with 20 mM and 40 mM imidazole steps and proteins were eluted using 250 mM imidazole in 50 mM Tris-HCl, 100 mM NaCl, 0.5 mM MgCl 2 (pH 7.5)

The effects of increased/decreased O-GlcNAcylation levels, depending on the treatment with the aforementioned modulators, were evaluated using an anti-O-GlcNAc antibody (Rl2)

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10.1677/joe.0.1550351

Thus, neuroinvasion by SARS-CoV-2 is possible, providing activation of reactive astrogliosis, triggering increased production and secretion of cytokines and other pro-inflammatory factors, and perpetuating neuroinflammation (Daniels et al