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Description
You may start to experience gastrointestinal symptoms such as abdominal discomfort, indigestion, diarrhea, and bloating
It depends on what you are treating
in vivo not known at level of ER, but saturating levels of 25-40 mM use in vitro with unactivated microsomes and 2-10 mM with activation (e.g

Hydroxy B 12 is generally safe

You attached a his-tag onto your protein of interest while cloning it

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