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Xu, X., Wang, S., Cheng, J

This research was funded by a grant from Seoul National University Hospital Korea [grant number: 800-20200291]

Each approach has significant drawbacks

I consistently recommend completing at least the minimum protocol duration even when subjective improvement comes early

The lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

However, analyzing liquid substances with mass spectrometry required vaporization and ionization, presenting limitations to liquid chromatography-mass spectrometry (LC-MS) until the advancement of atmospheric pressure ionization (API) technology