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doi: 10.1515/bmc-2012-0045

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Secondary antibodies (AlexaFluor 594 anti-mouse 1:500 (Invitrogen, A11005) and AlexaFluor 488 anti-rabbit 1:500 (Invitrogen, A11034)) were added to blocking buffer and incubated at room temperature for 1 h

Of the 60 patients who completed the study, those who used methylene blue oral rinse reported significantly reduced pain after two days of treatment

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