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Gut Microbiota Composition in Depression Gut microbiota composition has been increasingly recognized as a significant factor in the pathology of depression

If they continue, it might not be the biotin

For protein digestion, 0.5 g of trypsin (Promega) was added to the samples, followed by an overnight incubation at 37 C

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Lentivirus was produced in 293T cells using Lipofectamine 2000 (9 g packaging plasmid psPAX2 (Addgene 12260), 0.9 g envelope plasmid pCAG-VSVG (Addgene 35616), 9 g scramble control or shLDHB vectors (Origene TL311768) in 225 L Opti-MEM (Thermo Fischer 31985062) mixed with 90 L Opti-MEM containing 54 L Lipofectamine 2000, 10-cm dish), the medium was changed after 18 h of incubation, then the virus was collected 24 and 48 h later

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