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After reconstitution: keep refrigerated and use within the period indicated on the product label

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Prior to experimentation, the enzyme was thawed at room temperature and small amounts of precipitated enzyme were removed by centrifugation at 10,000 g for 10 min, and 30 kDa centrifugal filters were used to buffer exchange the supernatant enzyme into the desired reaction buffer (typically 50 mM KPi, pH 7.4)

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Firstly, EGT can not only prevent the formation of free radicals such as OH, but also directly scavenge free radicals and reactive oxygen species (ROS) such as hypochlorite acid (HClO) and peroxynitrite (Rougee et al., 1988