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Paired statistical comparisons using the Wilcoxon signed-rank test identified nominally a statistically significant changes in coumarin , L-leucine , and 5-methoxytryptamine (Fig

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Subsequently, 5 ml of bacterial cultures ( oxyR-msrA , oxyR-msrB or no plasmid) at an OD 600 of 0.5 were mixed with 5 ml of NFs in a clean microcentrifuge tube and incubated at 37 C, 160 rpm throughout the experiment

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This occurs due to increased melanin production in the iris , which may lead to a darker, more brown-toned appearance over time