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After aspiration and washing, 100 l of substrate solution was added to each well and incubated for 30 min at room temperature. Then, 100 L of a stop solution was added, and color changes were measured at 450 nm using a BioTek ELISA reader

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doi: 10.1016/s1079-2104(98)90173-1 84 WuFWengSLiCSunJLiLGaoQ

Sources: Takeuchi S, et al

Preventing Complications: Repairing the hernia can prevent complications like bowel obstruction or tissue damage, ensuring long-term comfort and health
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