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By quantifying how much of your protein was in your elution, and analyzing how much, if any, of your protein was in the flow-through, you can figure out if you used an appropriate amount of glutathione resin

Then, a fermentation experiment in Erlenmeyer flasks was performed using the G3-SF strain (containing ScGSH1, ScGSH2, StGshF, and ScADK1), with the highest GSH titer and yield of 999.3347.26 mg/L and 91.534.70 mg/g, respectively

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