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[Abstract]2022 Sep;150(3):727-735.e6

doi: 10.1096/fj.202201400R 29

Nabi T, Nabi S, Rafiq N, Shah A

Femurs and tibias were removed and cells were isolated from bone marrows by fractionation into discontinuous Percoll (Sigma) gradients (80%, 65%, 55%)

Rahman, I., Bel, A., Mulier, B., Donaldson, K

Enrichment of phosphopeptides by Fe immobilized metal ion affinity chromatography (IMAC) The remaining 95% was concatenated into 12 fractions and subjected to Fe 3+ -IMAC phosphopeptide enrichment using Fe 3+ -conditioned Ni-NTA beads
