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Total RNA was isolated from the frozen biopsies using TRIzol reagent (Invitrogen, Carlsbad, USA) and purified with the RNeasy Mini Kit (Qiagen, Venlo, the Netherlands)

Potassium replacement therapy should be administered if necessary

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Interestingly, treatment withdrawal did not completely restore the mRNA transcripts, and a similar effect was observed for vitamin D metabolism genes and genes associated with NAFLD which was persistently higher, suggesting the epigenetic association between GSH deficiency and vitamin D metabolism genes

There is a large body of literature on the prevention of medication errors within health care institutions, but there is a paucity of evidence to guide us in how to reduce medication errors in the outpatient setting