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In vitro, inflammation can be achieved by a variety of methods, including the use of MPTP or LPS for induction

The RNAscope Multiplex Fluorescent Reagent Kit V2 (Advanced Cell Diagnostics #323100) was used to perform fluorescent labeling of the indicated RNA molecules according to the kit manual
Involvement of nuclear factor kappaB in up-regulation of aldose reductase gene expression by 12-O-tetradecanoylphorbol-13-acetate in HeLa cells

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