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Such blind injections can be effective, but may miss the mark in some cases

The original separation membrane is formed on ER from locally synthesized lipids, then an increase in the biosynthesis of the bilayer-forming phospholipids [phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylserine (PS)] occurs simultaneously with the induction of autophagy (Figure 2)
SandholmNColeJBNairVShengXLiuHAhlqvistEet al

J.SharadJ

During autophagy, ATG12-ATG5 coupling plays a key role in the lipidation of LC3
