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Cell lysates were boiled for 15 min and protein concentration was determined by the Lowry method

Suwannakul, N

L., Athanasou, N

Thus, the clinical evolution of small-cell carcinoma is more aggressive, exhibiting high metastatic, invasive, and angiogenic potential [48,49]

2.8.1 Sequencing sample pretreatment The appropriate amount of sample (between 0.20.5 g) was removed from the refrigerator and added to the centrifuge tube containing the extract lysate for grinding