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Its metabolite, kynurenine, suppresses Teff function while promoting Treg differentiation through aryl hydrocarbon receptor (AhR) activation

However, its clinical application has been limited by dose-dependent gastrointestinal toxicity observed in later phase I and II clinical trials

78 (6): 44953 Quigley HA, Nickells RW, Kerrigan LA, et al

Confocal microscopy For confocal microscopy assay, cells were washed twice with a washing buffer (D-PBS supplemented with 4.5 g/L of glucose and 5 mM of MgCl 2 ), followed by treatment with Cy5-labeled AsiG (250 nM) either free or loaded by liposome with or without anti-CD71 antibody in a binding buffer (D-PBS supplemented with 4.5 g/L of glucose, 5 mM of MgCl 2 , 1 mg/mL BSA, and 0.1 mg/mL of yeast tRNA) for 1 h at pH 7.4 or 6.5 on ice

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doi: 10.1128/MCB.00601-09