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These radicals then oxidized 3, 3, 5, 5-tetramethylbenzidine (TMB), resulting in the formation of the blue oxidized product oxTMB, observed a visible color change (from colorless to blue), and oxTMB had an obvious absorption peak at 652 nm

Plan: 68 sessions, every 710 days

And definitely dont add any glutathione to your binding buffer save that for the elution step

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