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80 The second study was a cross-sectional study 81 including antiretroviral therapy (ART)-nave adults

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Thereafter, horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Jackson Immunoresearch, West Grove, PA) was diluted in PBS+1%BSA to a final dilution of 1:1,000, and 50 L of this dilution was added to each well in the microtiter plates