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[PMID: 32015507 DOI: 10.1038/s41586-020-2012-7] [DOI] [PMC free article] [PubMed] [Google Scholar] 32.Wu C, Chen X, Cai Y, Xia J, Zhou X, Xu S, Huang H, Zhang L, Zhou X, Du C, Zhang Y, Song J, Wang S, Chao Y, Yang Z, Xu J, Zhou X, Chen D, Xiong W, Xu L, Zhou F, Jiang J, Bai C, Zheng J, Song Y

Hewson-Hughes et al., 2016)

Lancet Neurol 12: 822838, 2013

Borasio GD, Voltz R, Miller RG

The active electrode was positioned on the forehead, the reference electrode was placed beneath the left ear pinna, and the ground electrode was positioned under the right ear

However, if you notice that your protein is aggregating after elution with any of these affinity tags, then it may be worth trying a different tag to avoid that particular eluent
