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Each year, 5 to 35 out of every 100,000 people are diagnosed with PD, and its global prevalence for 2050 has been projected to reach 230 to 320 cases per 100,000 [1, 2]

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Motor neuron differentiation and maintenance The iPSCs were seeded on Matrigel-coated dishes in E8 (Gibco) medium and maintained in feeder-free conditions for at least 2 passages prior to differentiation