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Phomazines AC ( 59 61 ) were isolated from Phoma sp

Membranes were incubated in blocking solution [5% (w/v) non-fat dry milk in TTBS [25 mM Tris/HCl (pH 7.5)/0.15 M NaCl/0.05% (v/v) Tween 20], for 1 h at room temperature with shaking

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However, three overdose patients had APAP-CYS concentrations that were of the same order of magnitude as those observed with therapeutic dosing