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Fluorescence microscopy Worms were immobilized with 3 mM levamisole and mounted on 2% agarose pads on a microscopic slide

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Instead, NAC is used to provide cysteine in a stable supplement form

22 Therefore, to determine whether the metabolite induces proteotoxicity in part through UPS inhibition, we reduced proteasome function with a range of MG132 concentrations and identified that (at concentrations above 5 M) MG132 induces neurodegeneration in the presence of -synuclein and that this neurotoxicity epistatically regulates metabolite-induced neurotoxicity (Supplementary Figure S4a)

10.1016/j.jconrel.2020.04.026 83 ZhangX.ChenX.JiangY.-W.MaN.XiaL.-Y.ChengX.et al (2018)

Nrf2 is the key transcription factor required for activation of ARE