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Collagen

For intracellular staining of hexokinase-1, cells were stained for extracellular markers, fixed in 4% FA at RT, and stained with Anti-Hexokinase 1 (Abcam ab184818) diluted 1:100 in PBS 0.1% Tween-20 at RT

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In LPS-stimulated BV-2 microglial cells, ISA can activate the GSK-3 and its downstream Nrf2 and HO-1, which suppressed the activation of NF-B and the subsequent production of inflammatory mediators, causing the inhibition of inflammation (Wang et al., 2018a)

Through lipidomics and RNA sequencing, Bi et al

They also have cancer prevention potential by clearing cancer risk factors (Table 4)