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Y., Li, H., Luo, X
While short-read sequencing technologies produce reads of up to 600 bases, long-read sequencing technologies produce reads in excess of 10 kb ( The technology employed by PacBio interrogates a single molecule of DNA in real time

Validation criteria should extend beyond basic analytical performance (sensitivity, specificity, and reproducibility) to include clinical practicality metrics, such as compatibility with routine clinical sample processing workflows, rapid matrix application protocols, and scalability for large-scale clinical cohorts

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As with all other pigmented lesions, a biopsy is warranted if a diagnosis cannot be appropriately rendered
