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Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering

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1 Researchers have observed that AD patients also have significantly depleted levels of glutathione, reducing protection from oxidative stress

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83 NiciuM

First, the sgRNAs [ Mpst upstream (target sequence: 5GGAGAGACAAGCCTGCATTC AGG 3) at intron 1 and Mpst downstream (target sequence: 5GTGGGTGGCGGAGGCTCTGA AGG 3) at exon 2, where the underlined bases indicate the PAM sequences] were synthesized in vitro (T7 gRNA Smart Nuclease Synthesis Kit, System Biosciences, Mountain View, CA, USA) following the manufacturer's instructions