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Description
This stability is relevant to research protocol design, particularly in protocols involving extended storage or varied conditions
Lately, two additional FLAG sequences were added in tandem to the original FLAG tag, the triple FLAG tag (3 FLAG), which showed an increased detection limit of the target protein, favoured binding to the M2 antibody, and the possibility to recover the protein by competitive elution with 3 FLAG peptide (Hernan et al., 2000)

doi: 10.1158/0008-5472.CAN-15-1208

C.et al (2012)
4D-printed matrices enable shape-memory and stimuli-responsive degradation, while biosensor-integrated dressings unite therapeutic release with continuous monitoring of ROS, pH, and temperature

Pragmatics - helping individuals understand when and how to use language socially
