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According to the elevated, filterable free copper, urinary copper is increased without further stimulation by D-penicillamine, making it a very reliable diagnostic parameter [1,4] [Table 2]

Some clients prefer it for this reason

J Surg Res 2001

and B.J

Hematoxylin staining was performed for 5 min, differentiated with hydrochloric acid for 2 s, blued with ammonia water for 1530 s, and washed with water
