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During the Civil War, Peck enlisted at New York City as a first lieutenant on June 20, 1863, was commissioned into the 4 th Regiment on July 18 as a first lieutenant and adjutant, and mustered out on July 24 of that year at New York City

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So, you would have already purified proteins corresponding to the full-length and each individual domain of the interacting protein, and now you do a pull-down to see which ones bind

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