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Sequencing, data processing, and analysis parameters The study's primary aim was to determine whether 5A-1MQ treatment in combination with a switch from WD to LD rendered the intestinal microbiome comparable to that of mice continuously maintained on LD, and distinct from that of DIO mice switched to LD but given saline injections or mice maintained continuously on WD

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After centrifugation at highest speed in a microcentrifuge, one microliter of supernatant was used for PCR amplification

Existing in all the organelles, the AsA-GSH pathway protects the cell from the toxic effects of ROS generated under a variety of abiotic and biotic stresses (Anjum et al., 2010) (Figure 2)

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