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In this context, physical, chemical, and microbiological approaches are applicable

Januszewski J, Forma A, Zembala FM, Tyczynska M, Dring JC, Dudek I, et al

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The primary and secondary antibodies were diluted in a PBS solution containing 3% BSA, 1% horse serum, and 0.1% Triton X-100

Zhang M, Atkinson RL, Rosen JM