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Description
Briefly, after a 1:10 dilution of the plasma samples in UltraPure TM DNase/RNase-Free H2O (Invitrogen, Corporation, CA, United States), a pre-validated qPCR assay was used to amplify a hominoid specific repetitive element, which allows sensitive and reliable quantification of cfDNA in low amounts of plasma samples
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Every 4 weeks for the first 12 weeks, then every 12 weeks thereafter
