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(7) Centrifuge for 10 min (12000 rpm, 4C), 150 L of supernatant was loaded into an LC-MS injection vial with a liner tube for LC-MS analysis
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LSM7 DUO) using ZEN 2009 software (Carl Zeiss)
This loss of collagen and epithelial stiffness is also suggested to be a cause of other diseases and aging-related complications such as corneal 130 , skin 131 and teeth tissue 132 deteroriation
Seip HM, Alstad J, Carlberg GE, Martinsen K, & Skaane R (1986) Measurement of mobility of organic compounds in soils
