what is glutathione protein Glutathione? Protein Glutathionylation and Glutaredoxin: Role

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Figure 2. Retained His-tagged protein binding capacity in presence of EDTA. Ni-Penta 6FF resins were added to 20 volumes of E.coli lysate (~0.5 mg/mL containing a His-tagged protein of 26 kDa in size), in the presence of 2 mM, 5 mM, 10 mM EDTA respectively. Protein binding was then performed at room temperature for 2 hours. After washing 5 times with 20mM sodium phosphate, 0.5 M NaCl, 5 mM imidazole, pH7.4, the target His-tagged protein was eluted with 20mM sodium phosphate, 0.5 M NaCl, 250 mM imidazole, pH7.4. Protein concentration was measured by UV absorbance at OD280

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[Google Scholar] 77.Mani DP, Singh D

[DOI] [PMC free article] [PubMed] [Google Scholar] Curthoys NP, Watford M

Effects of tea polyphenol combined with nisin on the quality of weever (Lateolabrax japonicus) in the initial stage of fresh-frozen or chilled storage state