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We repeated this experiment where 24 h after seeding, cells were pre-treated with DMSO (0.003% v/v) control or FOXO1 inhibitor (1 M) for 12 h in full media conditions

The status of glutathione is considered the single most accurate indicator of the health of the cell

It plays a vital role in protecting cells from oxidative stress, neutralising free radicals and supporting skin repair

Thus, tissue samples (from both tumor and healthy sections of the incised lung piece) were preserved from post-sampling oxidation immediately upon collection in neutrally buffered 80% methanol containing the cysteine alkylating reagent N-ethylmaleimide (NEM) following our recently published method 15 , enabling an unbiased determination of both small molecular weight (e.g., glutathione) as well as protein thiols

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doi: 10.1667/RR15362.1
