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Di-(2-ethylhexyl) phthalate (DEHP) at specific concentrations can produce neurotoxic effects after prenatal and postnatal exposure

Determination of SOD activity The total activity of SOD was measured in the supernatant of heart tissue homogenate by the Cayman Chemical SOD Assay kit (USA) which utilizes a tetrazolium salt to detect the generated superoxide radicals by measuring the absorbance at 420 nm

Suzuki et al

Hu G et al

(7) Centrifuge for 10 min (12000 rpm, 4C), 150 L of supernatant was loaded into an LC-MS injection vial with a liner tube for LC-MS analysis

Each pathway involves two one-electron transfers, highlighting the nuanced role of distal residues in facilitating these reactions and emphasizing the atomic and electronic reorganizations crucial to the catalase mechanistic pathway [47]