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Collectively, data show that elevated plasma TMAO levels contribute to inflammatory and cardio-metabolic risks via the induction of inflammatory cytokines [72,73]

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Plasma level of TMAO is determined by a number of factors including diet, gut microbial flora and liver flavin monooxygenase activity

Relative expression was determined by the 2 Ct method using the housekeeping gene, GAPDH, as an internal control, and the fold change was calculated by its comparison with the corresponding control group [28]