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K-means clustering analysis of amplicon sequence variants by animal demonstrated that NNMTi-treated DIO mice switched to LD had a distinct microbiome pattern that was highlighted by decreased Erysipelatoclostridium and increased Lactobacillus relative abundances compared to vehicle counterparts

No IGF-1 or glucose disruption: Unlike HGH, AOD-9604 does not significantly increase IGF-1 or negatively affect insulin sensitivity, making it a safer candidate for metabolic regulation

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