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After rinsing with PBS, all sections were incubated at room temperature for 2 h with IgG goat anti-rabbit (Thermo Fisher Scientific, Alexa Fluor 488) diluted 1:200 in 0.01 M PB containing 1% BSA and 0.3% Triton-X100

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The incidence of this behaviour is increasing ..This appears to be due to a cost saving exercise and largely happens without any prior consultation with individual patients to discuss their needs

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The most common side effects include nausea (occurring in up to 30% of users at higher doses), diarrhea, constipation, decreased appetite, vomiting, and abdominal pain