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Before each iron infusion therapy, your blood iron levels are measured in order to dose the infusion according to your needs

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Using LR Clonase II Plus (Thermo Fisher Scientific), the specific pMuLE ENTR U6-sgRNA and pMuLE ENTR CMV-hCas9 L5-L2 plasmids were recombined with SleepingBeauty transposon plasmid pMuSE eGFP-P2A-PuroR-DEST ( 86 )